Journal: iScience

Article Title: Newt regeneration genes regulate Wingless signaling to restore patterning in Drosophila eye

doi: 10.1016/j.isci.2021.103166

Figure Lengend Snippet:

Article Snippet: The primary antibodies used were mouse anti-phospho histone-3 (1:200) (Cell Signaling, Catalog no. 9706); Rabbit anti- Drosophila caspase-1 (DCP-1) (1:100) (Cell Signaling, Catalog no. 9578); rat anti-Elav (1:100) (Developmental Studies Hybridoma Bank, Catalog no. 7E8A10); mouse anti-Wg (1:100) (Developmental Studies Hybridoma Bank, Catalog no. 4D4), and goat anti-Hth (a gift from H. Sun and R. Mann).

Techniques: Plasmid Preparation, Electron Microscopy, Bicinchoninic Acid Protein Assay, TUNEL Assay, RNA Sequencing, Gene Expression, Software

Journal: The Journal of Biological Chemistry

Article Title: cGMP/Protein Kinase G Signaling Suppresses Inositol 1,4,5-Trisphosphate Receptor Phosphorylation and Promotes Endoplasmic Reticulum Stress in Photoreceptors of Cyclic Nucleotide-gated Channel-deficient Mice *

doi: 10.1074/jbc.M115.641159

Figure Lengend Snippet: List of antibodies used in this study

Article Snippet: All other reagents were purchased from Sigma, Bio-Rad, and Invitrogen. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Provider Catalog no. Dilutions used in immunoblotting (IB) or immunofluorescence (IF) labeling M-opsin Dr. Cheryl Craft, Keck School of Medicine, University of Southern California 1:2000 (IB) S-opsin Dr. Muna Naash, University of Oklahoma Health Sciences Center 1:1000 (IB) Cone arrestin Dr. Cheryl Craft, Keck School of Medicine 1:2000 (IB) GADD 153 (CHOP-10) Santa Cruz Biotechnology Inc., Santa Cruz, CA sc-575 1:100 (IB) Phospho-CREB sc-7978 1:500 (IF) Phospho-eIF2α Cell Signaling Technology, Beverly, MA 3398 1:500 (IB) Phospho-IP 3 R 3760 1:250 (IB) Caspase-7 9492 1:250 (IB) Histone 3 (H3) 4499 1:2000 (IB) ATF-6 Active Motif, Carlsbad, CA 40,962 1:250 (IB) GFAP Dako Denmark A/S, Glostrup, Denmark Z0334 1:500 (IF) β-Actin ab-6276 1:2000 (IB) Phospho-IRE1α Abcam, Inc., Cambridge, MA ab-48187 1:100 (IB) Open in a separate window List of antibodies used in this study

Techniques: Western Blot, Immunofluorescence, Labeling, CRAfT Assay